Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells.

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Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells

Ionic currents induced by 5-hydroxytryptamine (5-HT) in cultured neuroblastoma N18 cells were studied using whole-cell voltage clamp. The response was blocked by 1-10 nM 5-HT3 receptor-specific antagonists MDL 7222 or ICS 205-930, but not by 1 microM 5-HT1/5-HT2 receptor antagonist spiperone or 5-HT2 receptor-specific antagonist ketanserin. These 5-HT3 receptors seem to be ligand-gated channels...

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J on channels are pore-forming macromolecules that provide a passive conduit for ion transfer across membranes. Although simple ionophores such as gramicidin and nystatin' fall within this legalistic definition, the ion channels of eukaryotic cells are integral membrane proteins, many of which have now been isolated biochemically, reconstituted, cloned, and expressed. These fascinating proteins...

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Mechanism of Ion Permeation in Mammalian Voltage-Gated Sodium Channels

Recent determination of the crystal structures of bacterial voltage-gated sodium (NaV) channels have raised hopes that modeling of the mammalian counterparts could soon be achieved. However, there are substantial differences between the pore domains of the bacterial and mammalian NaV channels, which necessitates careful validation of mammalian homology models constructed from the bacterial NaV ...

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Ca2F permeation in cyclic nucleotide-gated channels

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Ca2+ permeation in cyclic nucleotide-gated channels.

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1990

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.96.6.1177